LightOx™14

≥98% (HPLC)
Retinoic Acid Analogue

Biochemical/Physiological Actions

Strong ligand for retinoic acid receptor (RAR) proteins

Applications

• Fluorescence microscopy
• Fluorescent retinoid binding assays
• Fluorescence spectroscopy

Features and Benefits

LightOx14™ is a synthetic analogue of all-trans-retinoic acid (ATRA) that exhibits solvatochromatic fluorescence emission when excited with UV 405 nm light. The compound is a strong ligand for the retinoic acid receptor proteins (RARs) and related cellular retinoic acid binding proteins (CRABPI/II) and can, therefore, be used as a replacement for ATRA in cellular assays involving retinoid signalling processes.

LightOx14™ can be utilised to study retinoid behaviour using fluorescence microscopy, employed in a competitive fluorometric ligand-binding assay for direct determination of the strength of binding of non-fluorescent retinoids to a protein target, and as a fluorophore in fluorescence spectroscopy.

Papers

LightOx™14 is referred to as DC271 in the following papers

Fluorescent Retinoic Acid Analogues as Probes for Biochemical and Intracellular Characterization of Retinoid Signaling Pathways

Novel Fluorescence Competition Assay for Retinoic Acid Binding Proteins

A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

Genomic and non-genomic pathways are both crucial for peak induction of neurite outgrowth by retinoids